Abstract
The patch clamp technique can perform intracellular recording of neurons, but is not well suited for parallel recording; an array of microelectrodes can do parallel recording, but they are too large to access the interior of live neurons. In fact, no methods currently available are capable of both intracellular and parallel recording of mammalian neurons, while such dual ability can offer new possibilities in neurotechnology as well as fundamental neuroscience. Nano-bio interface may be one way to tackle this issue, as recently shown by co-PI, Park [Nature Nano. 7, 180 (2012)]: nanoelectrodes can penetrate into live neurons, acquiring intracellular access; an array of these nanoelectrodes––whose construction is possible via standard top-down fabrication––can then enable intracellular and parallel recording. Here we will develop a CMOS integrated circuit with an array of nanoelectrodes on top. The CMOS electronics (an array of analog amplifiers and digital control electronics) right below the nano-bio interface array will facilitate parallel operation of nanoelectrodes, and increase the recording sensitivity. The high impedance of nanoelectrodes and low-frequency sub-threshold neuronal signals pose a unique challenge for the semiconductor circuit design. Subsequently, we will use this unprecedented electrophysiological tool for pharmaceutical screening for neurological disorders and cellular-level neuroprosthesis.
Publications
- J. T. Robinson, M. Jorgolli, A. K. Shalek, M.-H. Yoon, R. S. Gertner, and H. Park, “Vertical nanowire electrode arrays as a scalable platform for intracellular interfacing to neuronal circuits,” Nature Nanotech., vol. 7, no. 3, pp. 180–184, 2012.
- J. T. Robinson, M. Jorgolli, and H. Park, “Nanowire electrodes for high-density stimulation and measurement of neural circuits,” Front. Neural Circuits, vol. 7, no. 38, 2013.
- J. Abbott, T. Ye, D. Ham, and H. Park, “Optimizing Nanoelectrode Arrays for Scalable Intracellular Electrophysiology,” Acc. Chem. Res., vol. 51, no. 3, pp. 600–608, Feb. 2018.
- J. Abbott, T. Ye, L. Qin, M. Jorgolli, R. S. Gertner, D. Ham, and H. Park, “CMOS nanoelectrode array for all-electrical intracellular electrophysiological imaging,” Nature Nanotech., vol. 12, no. 5, pp. 460–466, Feb. 2017.
- J. Abbott, L. Qin, T. Ye, M. Jorgolli, R. S. R. S. Gertner, H. Park, and D. Ham, “CMOS electronics probe inside a cellular network – Invited review paper,” in 2018 IEEE Custom Integrated Circuits Conference, CICC 2018, 2018, pp. 1–7.
- J. Abbott, T. Ye, K. Krenek, R. Gertner, S. Ban, Y. Kim, L. Qin, W. Wu, H. Park, and D. Ham, “Intracellular Recording of Thousands of Connected Neurons,” submitted 2018.
Report
Link to PDF: Final Report